| Products/Services Used | Details | Operation |
|---|---|---|
| Catalog Antibody | … Materials and methods Plant treatments, RNA and quantitative Real Time PCR (qRT-PCR) Leaf tissue was collected from 4-week old Arabidopsis plants that were syringe-infiltrated with 10 mM flg22 (Genscript) or 10 mM elf18 (Genscript) … | Get A Quote |
The WRKY proteins belong to a superfamily of TFs that play pivotal roles in responses to a wide range of biotic， abiotic， developmental and physiologic cues. Here， we assayed the accumulation of basal WRKY27 transcripts in diverse tissue including root， shoot， leaf and flowers. We demonstrated that plants over-expressing WRKY27 transcript levels exhibit growth aberrations and fertility defects. Scanning electron microscopic data suggest that WRKY27 overexpressor plants exhibit pollen dehiscence defects. Our fluorescein diacetate hydrolysis assay showed that flowers of plants overexpressing WRKY27 display significantly decreased pollen viability. These sterility-related phenotypes were not rescued by t... More
